BDNF ELISA Kit (Chicken)

Cat# OKEH03797

Size : 96T

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityChicken, Gallus gallus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BDNF ELISA Kit (Chicken) (OKEH03797) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BDNF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BDNF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BDNF captured in well.
ELISA Kit Range0.625-40ng/ml
ELISA Kit Component
ComponentAmount
Anti-BDNF Microplate96 Wells (12 x 8 Well strips)
BDNF Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated BDNF Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.273 ng/mL
SpecificityNatural and recombinant Chicken Brain-derived neurotrophic factor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBDNF
Gene Full Namebrain derived neurotrophic factor
Alias Symbolsbrain-derived neurotrophic factor;neurotrophic factor;pre-pro-brain-derived.
NCBI Gene Id396186
Protein NameP25429
Description of TargetBrain-derived neurotrophic factor
Uniprot IDhttps://www.uniprot.org/uniprot/P25429
Protein Accession #NP_001026787.1
Nucleotide Accession #NM_001031616.1
Protein Size (# AA)246
Molecular Weight28 kDa