TNFB ELISA Kit (Cattle)

Cat# OKCD04431

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for TNFb ELISA kit (Bovine)
Product Info
Predicted Species ReactivityBovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)92-101%93-101%82-101%87-95%
EDTA Plasma (n=5)82-93%79-91%92-105%82-91%
Heparin Plasma (n=5)88-97%85-93%81-95%95-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Beta (TNFb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Beta (TNFb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Beta (TNFb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tumor Necrosis Factor Beta (TNFb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)81-9087
EDTA Plasma (n=5)78-9585
Heparin Plasma (n=5)78-10492
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Beta (TNFb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Beta (TNFb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Tnfb Microplate96 Wells (12 x 8 Well strips)
Tnfb Lyophilized Standard2
100X Tnfb HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Sensitivity0.059 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTNFB
NCBI Gene Id280845
Protein Namelymphotoxin-alpha
Uniprot IDQ06600
Protein Accession #NP_001013419.1
Nucleotide Accession #NM_001013401.2