MPO ELISA Kit (Cattle)
Cat# OKCD04236
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for MPO ELISA Kit (Cattle) (OKCD04236) |
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| Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloperoxidase (MPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloperoxidase (MPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloperoxidase (MPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myeloperoxidase (MPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 1.56-100ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloperoxidase (MPO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloperoxidase (MPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.62 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |