Ultra-fast DNA library preparation kits for Illumina® - Transposase

Ultra-fast DNA library preparation kits for Illumina® - Transposase

A transposase is a nucleotidyltransferase that catalyzes the movement of a transposable element (transposon) from one place in the genome to another. This enzyme binds to one end of the transposon, cleaves the bond between the transposon and the rest of the genome, and sutures the transposable element to another location in the genome.

Tagmentation is an enzymatic process that is the initial step in the preparation of the library where the unfragmented DNA is cleaved and labeled for analysis in a single step.

1. Tagmentation of DNA
 DNA is fragmented by an enzyme called tagmentase. The tagmentatase is a modified transposase: a mixture of a transposase and a transposon end complex that forms the transposome. Tagmentation consists of DNA fragmentation and the simultaneous addition of Illumina® sequencing adapters.
Tagmentation technology eliminates the various purification and ligation steps required for the conventional method.
We offer different kits for which the tagmentation step has been optimized for different amounts of initial DNA (50 ng, 5 ng and 1 ng).

2. Amplification by cycle-limited PCR
In this second step, the fragmented DNA is amplified by cycle-limited PCR, using primers targeted to the transposon sequence. These primers add the specific adapters and barcodes (if desired) to the fragments.

Tagmentation technology therefore significantly reduces library preparation time and the initial amount of DNA.