Phytopathology reagents - Guaiacol

Phytopathology reagents - Guaiacol


Guaiacol is commonly used in colorimetric assays to measure peroxidase enzyme activity. Initially colorless, guaiacol undergoes polymerization in the presence of a peroxidase and H2O2 to form a brown compound known as tetraguaiacol which can be measured spectrophotometrically at roughly 470 nm (Johannes, 2006; Doerge, 1997; Balešević-Tubić, 2011). Guaiacol has also been documented to detect Helminthosporium oryzae and Trichoconis padwickii in rice seeds when incorporated into an agar medium with streptomycin by staining the surrounding medium red in the presence of the fungus (Kulik, 1975). Medium components according to Kulik (1975) are 0.125g guaiacol, 5g agar & 0.5g streptomycin (added after autoclaving) per liter of water. In soybean seed testing, guaiacol separates different cultivars based on high and low seed coat peroxidase activity. The level of peroxidase activity of the seed coat is based on the color change of the guaiacol (Schmitz, 1997).

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Description
Cond.
Price Bef. VAT
NB-42-01370
 Onrequest 
T1301-20mg
 20mg 
T1301-1mL
 1mL 
T60493-500mg
 500mg 
332-10500-4
 200mg 
332-10500-3
 100mg 
T21072-500mg
 500mg 
T21072-100mg
 100mg 
T36594-500mg
 500mg 
TRC-G245525-50MG
 50mg 
300388-20mg
 20mg 
TRC-G245582-0.5MG
 0.5mg 
T40180-5mg
 5mg 
TRC-G245582-2.5MG
 2.5mg 
TRC-G245582-5MG
 5mg 
TRC-G245527-25MG
 25mg 
284946-25g
 25g 
284946-5g
 5g 
284946-10g
 10g