Mix for probes for RT-qPCR
Real-time quantitative RT-PCR based on fluorescent probes relies on the specific detection of a sequence of a desired PCR product. Unlike methods based on SYBR green that detect all double-stranded DNAs, quantitative PCR using probes uses a fluorescently labeled specific probe, resulting in increased specificity and sensitivity. In addition, a variety of fluorescent dyes are available so that several primers can be used to simultaneously amplify many sequences.
Quantitative RT-PCR using fluorescent probes is therefore ideal for high-throughput studies. The ready-to-use mixes contain all the necessary components for qPCR, simply add fluorescent detection probes, primers and DNA to be amplified.
Many fluorescent sources based on primers and probes for qPCR have been designed, including:
- Hydrolysis Probes (TaqMan)
- Molecular markers
- Dual Hybridization Probes (FRET)
- Eclipse Probes
- Amplifluor assays
We propose a probe synthesis service for qPCR
Here's how to select the appropriate ROX dye for the qPCR device you are using:
|Without ROX||Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4;|
Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s;
Illumina Eco qPCR;
Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000;
Roche Applied Science LightCyclerTM 480;
Thermo Scientific PikoReal Cycler.
|Low ROX||Applied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000,|
|High ROX||Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast;|