IHC buffers - Quenching - AP

IHC buffers - Quenching - AP


Chromogenic detection methods often use a conjugated enzyme to visualize epitope-antibody interactions. When using this method of detection, the endogenous activity of the same enzyme must be blocked. For example, protocols that include horseradish peroxidase (HRP) or alkaline phosphatase (AP) may require reagents to prevent non-specific signals. Tissues such as kidney, liver, or vascular areas with red blood cells, contain endogenous peroxidase activity. Peroxidase blocking reagents formulated with 3-10% H2O2 can be used to prevent endogenous peroxidase from cleaving the substrate. Endogenous AP found in intestine, kidney, lymphoid and other tissue can be blocked with 1 mM Levamisole. The intestinal form of AP is unaffected by Levamisole but can be blocked by using 1% acetic acid.

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Cat#
Description
Cond.
Price Bef. VAT
285166-10mg
 10mg 
259114-50mg
 50mg 
002311-5mg
 5mg 
285166-100mg
 100mg 
259114-10mg
 10mg 
259114-100mg
 100mg 
285166-5mg
 5mg 
018241-10mg
 10mg 
259114-25mg
 25mg 
259114-250mg
 250mg 
285166-25mg
 25mg 
285166-50mg
 50mg 
50R-R9280
 50mg 
C178580-25mg
 25mg 
431362-25mg
 25mg 
431362-2.5mg
 2.5mg 
013928-2.5mg
 2.5mg 
AOB9361-1
 1mg 
AOB9361-25
 25mg 
AOB9361-50
 50mg