IHC buffers - Quenching - AP

IHC buffers - Quenching - AP


Chromogenic detection methods often use a conjugated enzyme to visualize epitope-antibody interactions. When using this method of detection, the endogenous activity of the same enzyme must be blocked. For example, protocols that include horseradish peroxidase (HRP) or alkaline phosphatase (AP) may require reagents to prevent non-specific signals. Tissues such as kidney, liver, or vascular areas with red blood cells, contain endogenous peroxidase activity. Peroxidase blocking reagents formulated with 3-10% H2O2 can be used to prevent endogenous peroxidase from cleaving the substrate. Endogenous AP found in intestine, kidney, lymphoid and other tissue can be blocked with 1 mM Levamisole. The intestinal form of AP is unaffected by Levamisole but can be blocked by using 1% acetic acid.

Cat#
Description
Size
Price Excl. VAT
431362-2,5mg
 2.5mg 
431362-25mg
 25mg 
L331103-100mg
 100mg 
L331103-10mg
 10mg 
L331103-250mg
 250mg 
L331103-50mg
 50mg 
N496400-100mg
 100mg 
N496400-10mg
 10mg 
N496400-25mg
 25mg 
N496400-50mg
 50mg 
259114-10mg
 10mg 
002311-5mg
 5mg 
259114-25mg
 25mg 
AOB9361-10
 10mg 
AOB9361-100
 100mg 
AOB9361-25
 25mg 
285166-10mg
 10mg 
AOB9361-5
 5mg 
285166-5mg
 5mg 
AOB9361-50
 50mg 
50R-R9280
 50 mg 
AOB0900-10
 10mg 
AOB0900-100
 100mg 
AOB0900-25
 25mg 
AOB0900-5
 5mg 
AOB0900-50
 50mg 
H943720-2.5mg
 2.5mg 
H943720-25mg
 25mg 
H943725-2.5mg
 2.5mg 
281321-10g
 10g 
281321-5g
 5g 
013927-2,5mg
 2.5mg 
013928-2,5mg
 2.5mg 
018241-10mg
 10mg 
015543-1g
 1g 
015544-1mg
 1mg 
MBS539911-50mg
 50 mg 
MBS575715-2mg
 2 mg 
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