HRP Chromogen for IHC - DAB

HRP Chromogen for IHC - DAB



DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).

Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling

Cat#
Description
Size
Price Excl. VAT
DAB (Post) Enhancing Solution
DES999
 1000 ml 
DAB Enhancer Solution
ACM030
 30 ml 
DAB Away Kit
ACP001
 500 ml 
orb219876
 60ml 
orb90435
 1Kit 
40480000-1
 5g 
40480000-2
 10g 
40480000-3
 25g 
40480004-1
 5g 
40480004-2
 10g 
40480004-3
 25g 
163582-5mg
 5mg 
PW023
 5 Preps 
CRG1005
 1 Kit 
D100040-50mg
 50mg 
D100040-5mg
 5mg 
OKRA00046
 1 Pack 
13060
 1G 
13080
 1G 
13082
 1PACK 
13083
 1PACK 
D1000-01-100g
 100g 
MBS176565-1
 1 Kit 
MBS176568-1
 1 Kit 
MBS545047-5g
 5 g 
MBS545047-25g
 25 g 
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