HRP Chromogen - DAB-Ni

HRP Chromogen - DAB-Ni



DAB (3,3'-Diaminobenzidine) is one of the most used substrates for peroxidase. Our DAB-Ni substrate is a DAB system enriched in nickel chloride with a higher sensitivity and contrast than conventional DAB. DAB-Ni forms a black deposit when it reacts with peroxidase.
The DAB-Ni substrate requires a 5 minute incubation in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When good staining is achieved, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB-Ni.
Like DAB, DAB-Ni is insoluble in alcohol and other organic solvents. Samples can be counter-stained, dehydrated and permanently mounted (O-Mount).

Name
DAB-Ni (3,3'-Diaminobenzidine + nickel chloride)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Black
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling

Cat#
Description
Size
Price Excl. VAT
NB-23-00151
 12 ml