Direct PCR on blood samples

Direct PCR on blood samples

Direct PCR kits on blood samples allow PCR to be performed directly on whole blood samples without DNA purification or pretreatment. These kits are compatible with different samples such as: fresh blood, blood stored at 4 ° C, frozen blood, blood stored on Whatman 903 paper or dried on FTA cards and are compatible with all conventional anticoagulants (EDTA, citrate , Heparin, etc.).
Silica membrane column purification methods can be used to remove contaminants and inhibitors, but are expensive and may have low yield. Less expensive approaches such as sodium hydroxide (NaOH) and proteinase K (PK) can save time, but contamination can produce inconsistent results. This is why it is preferable to use a direct PCR kit which combines the low cost of conventional extraction methods with the performance of DNA polymerases optimized for amplification from unpurified extracts.

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Cat#
Description
Cond.
Price Bef. VAT
NB-54-0162-02
 200rxn 
NB-54-0162-01
 50rxn 
AD401-02
 500rxns×20µlsystem 
AD401-01
 100rxns×20µlsystem 
G465
 5.0ml(200Rxns) 
G462
 100U(100μl) 
G463
 400U(400μl) 
MBS846807-5x100
 5x100Isolations 
MBS846807-100
 100Isolations