Classical rapid DNA polymerases
The speed of a PCR reaction may depend on a large number of factors, such as the degree of extension of the polymerase used, the speed of the thermocycler and the complexity of the sequence to be amplified. The levels of Taq DNA polymerase extension are around 1 kb / min whereas those of the enzymes used for rapid PCR are rather in the order of 2 to 4 kb / min.
Reduction of the PCR reaction time allows both time saving and the possibility of processing more samples. When using conventional DNA polymerase for fast PCR, this often results in a reduction of sensitivity and an increase in errors in sequence amplification. This is why it is important to use a DNA polymerase optimized for fast PCR in order to maintain the quality of the sequence while reducing the time necessary for its amplification.
We propose several DNA polymerases ideal for fast PCR.
|Name||TransFast Taq DNA polymerase||TransStart FastPfu
version of Taq DNA Polymerase
||Pfu DNA polymerase|
|Speed||6 kb/min||2-4 kb/min|
|Amplicon size||< 4 kb||< 20 kb|
|3'-5' exonuclease activity||Yes||Yes|
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