Classical High fidelity DNA polymerases

Classical High fidelity DNA polymerases

High fidelity PCR is frequently used for cloning and mutagenesis. However standard Taq polymerase is not sutable for this reaction, since it simply makes to many mistakes during amplification.
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Fidelity is important for applications in which the DNA sequence must be correct after amplification. 
The fidelity of a polymerase refers to its ability to insert the correct base during PCR. Conversely, the rate of misincorporation is known as a polymerase's error rate. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest.
Different types of high-fidelity DNA polymerases are distinguished, including Pfu polymerase, a naturally faithful DNA polymerase due to its 3'-5 'exonuclease activity as well as modified DNA polymerases to obtain higher fidelity capacities.
The modifications made to the DNA polymerases to give a higher fidelity are different: genetic modification, chemical modification, mixing of several enzymes ...

NameTaq Plus DNA polymeraseTransTaq DNA polymerase HiFiPhanta Max Super-Fidelity DNA polymerase
Product codeNB-03-0097 and NB-03-0099AP131P505
TypeMix of Taq polymerase and Pfu polymeraseMix of Taq polymerase and proofreading 3'-5' exonucleaseModified Pfu DNA polymerase
Speed0.2-0.4 kb/min1-2 kb/min1-2 kb / min
Amplicon size< 20 kb< 15 kb< 20 kb
Erro rate7.5x10-5 errors / nt3.6x10-6 errors / nt4.1x10-6 errors / nt
3'-5' exonuclease activityYesYesYes


Cat#
Description
Size
Price Excl. VAT
30-Z-309-2,500U
 2,5 KU 
30-Z-309-250U
 250 U 
30-Z-309-750U
 750 U 
P201-01
 250U 
P201-02
 1000U 
P201-03
 3000U 
orb94043-100U
 100units 
orb94043-500U
 500Units 
orb94046-100U
 100units 
orb94046-500U
 500Units 
orb90007-2500U
 2500Unit 
orb90007-250U
 250Unit 
orb90007-750U
 750Unit 
32-2837-750
 750 U 
D0090-1000U
 1000U 
D0090-200U
 200U 
OPPA00829
 250 U 
enz-309-2,500U
 2.5KU 
G012
 250 U (50 ul) 
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