Classical DNA polymerases for long fragments

Classical DNA polymerases for long fragments

The polymerase chain reaction (PCR) has been used extensively in the amplification of DNA fragments where there is a general size preference for products less than 1000 bp. Larger products may be synthesized at low or inefficient levels and then normally after considerable effort in reaction optimization. The maximum amplifiable length of PCR is limited by the low fidelity of the Thermus aquaticus (Taq) DNA polymerase, the most commonly used thermostable polymerase. This limitation is thought to be due to the error‐prone nature of the Taq polymerase, whereby those transcripts that end in an error (a misincorporated base) are not efficiently reinitiated and extended. 
Long range PCR allows the amplification of PCR products, which are much larger than those achieved with conventional Taq polymerases. Up to 27 kb fragments are possible from good quality genomic DNA. Amplification of long DNA fragments is desirable for numerous applications such as physical mapping applications and direct cloning from genomes.

Name Long Taq DNA Polymerase DNA polymerase I Large fragment
LAmp DNA polymerase
Product code NB-03-0110 and NB-03-0112 NB-15-1406 and NB-15-1407 P301
Type Mix of Taq polymerase and Pfu polymerase Cloned DNA Polymerase (klenow fragment) gene Mix of Taq DNA Polymerase and proofreading polymerase
Speed - - 1 kb/min
Amplicon size < 40 kb - > 20 kb
Error rate 3.6 x10-6 errors / nt - 4.1x10-6 errors / nt
3'-5' exonuclease activity Yes Yes Yes
HotStart No No No


List of the products

Cat#
Description
Size
Price Excl. VAT
Promotion
M1212-100
 500 U (100 ul) 
Promotion
M1213-200
 1600 U (200 ul) 
Promotion
M1214-200
 1000 U (200 ul) 
E2200-1000U
 1 KU 
E2200-250U
 250 U 
E2200B-1000U
 1 KU 
E2200B-250U
 250 U 
E039
 1.6 KU (200 ul) 
E043
 1 KU (200 ul) 
G460
 250 U (50 ul) 
G461
 1 KU (200 ul)