Classical DNA polymerases
Taq DNA polymerase is a thermostable DNA polymerase isolated from Thermus aquaticus.
This enzyme has a molecular weight of 95 kDa and exhibits both a 5'→3' DNA polymerase and a 5'→3 exonuclease activity. The 5'-3' exonuclease activity leaves a 3' overhang on the PCR product, which are convenient for direct t-A cloning.Taq DNA Polymerase lacks 3'-5' exonuclease activity and proofreading ability.
Taq DNA polymerase can withstand prolonged incubation at high temperatures without a significant loss of activity and is widely used in assays for molecular biology, like routine PCR and cloning PCR.
We offer a wide range of Taq DNA polymerase for use in PCR. Please find below a selection of Taq DNA polymerase with main features :
|Name||Taq DNA polymerase||EsayTaq|
|Product code||NB-03-0083 to 0087||AP111|
|Type||Taq polymerase||Taq polymerase|
|Speed||0.9-1.2 kb/min||1-2 kb/min|
|Amplicon size||< 5kb||< 4kb|
|5'-3' exonuclease activity||Yes||Yes|
|3'-5' exonuclease activity||No||No|