Biochemicals - IPTG

Biochemicals - IPTG


Isopropyl β-D-1-thiogalactopyranoside, abbreviated by IPTG, is a molecular biology reagent.

This compound is used as an analogue of allolactose, a lactose metabolite that activates the transcription of lactose operon and in particular the β-galactosidase gene, lacZ. Like allolactose or lactose, IPTG binds to the suppressor of the lactose operon, LacI, which prevents its binding to the operator and thus induces the transcription of the β-galactosidase gene. Unlike allolactose, IPTG is not a substrate for this enzyme because the presence of a sulfur atom in IPTG makes the chemical bond with the isopropyl part non-hydrolysable, so IPTG is what is called a free inducer. Not being metabolizable, the concentration of IPTG does not vary in the culture, which makes IPTG induction constant and stable.

In the laboratory, IPTG is used to induce the expression of recombinant proteins under the control of the lake promoter or its derivatives in Escherichia coli. Bacteria carrying a plasmid containing the gene of interest are cultured in the absence of IPTG until cell density reaches a sufficient level. The addition of IPTG to the culture allows the production of the protein to be induced at the desired time.

Cat#
Description
Size
Price Excl. VAT
NB-45-00030-25g
 25 g 
NB-45-00030-5g
 5 g 
NB-45-00030-5x5g
 25 g 
GF101-01
 1 ml 
HY-15921-S
 0,5 - 1mg 
HY-15921-5g
 5 g 
HY-15921-25g
 25 g 
HY-15921-10g
 10 g 
HY-15921-1g
 1 g 
orb340528-1g
 1 g 
orb340528-5g
 5 g 
HY-15921-1ml
 10 mM/1 mL 
I8500-05-5x10ml
 5x10ml 
I-1312-100g
 100 g 
I-1312-1g
 1 g  
I-1312-25g
 25 g 
G116
 1 g 
I-1312-50g
 50 g 
C0040
 5 g 
I-1312-5g
 5 g 
21530057-1
 1 g 
I1003-100GM
 100 g 
21530057-2
 2 g 
21530057-3
 5 g 
21530057-4
 10 g 
4026913.0005
 5 g 
21530057-5
 25 g 
4026913.0025
 25 g 
21530057-6
 50 g 
21530057-7
 100 g 
21530057-8
 250 g 
IB0168-100g
 100 g 
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