Biochemicals - IPTG

Biochemicals - IPTG


Isopropyl β-D-1-thiogalactopyranoside, abbreviated by IPTG, is a molecular biology reagent.

This compound is used as an analogue of allolactose, a lactose metabolite that activates the transcription of lactose operon and in particular the β-galactosidase gene, lacZ. Like allolactose or lactose, IPTG binds to the suppressor of the lactose operon, LacI, which prevents its binding to the operator and thus induces the transcription of the β-galactosidase gene. Unlike allolactose, IPTG is not a substrate for this enzyme because the presence of a sulfur atom in IPTG makes the chemical bond with the isopropyl part non-hydrolysable, so IPTG is what is called a free inducer. Not being metabolizable, the concentration of IPTG does not vary in the culture, which makes IPTG induction constant and stable.

In the laboratory, IPTG is used to induce the expression of recombinant proteins under the control of the lake promoter or its derivatives in Escherichia coli. Bacteria carrying a plasmid containing the gene of interest are cultured in the absence of IPTG until cell density reaches a sufficient level. The addition of IPTG to the culture allows the production of the protein to be induced at the desired time.

Cat#
Description
Size
Price Excl. VAT
NB-42-01685-5g
 5g 
NB-42-01685-25g
 25g 
NB-42-01685-10g
 10g 
HY-15921-1g
 1g 
HY-15921-5g
 5g 
HY-15921-1ml
 10mM*1mL 
HY-15921-25g
 25g 
HY-15921-10g
 10g 
HY-15921-500mg
 500mg 
IPTG
GF101-01
 1ml 
332-11828-1
 100mg 
332-11828-2
 500mg 
I747520-100mg
 100mg 
I747520-500mg
 500mg 
I747520-50mg
 50mg 
OORA01681
 1g 
IB0168-100g
 100g 
IB0168-1g
 1g 
IB0168-25g
 25g 
IB0168-5g
 5g 
G116
 1 g 
R3679-10g
 10g 
R3679-50g
 50g 
I1003-100GM
 100g 
I1003-25GM
 25g 
I1003-5GM
 5g 
10021
 100MG 
10021-1
 1G 
10021-2
 5G 
I-1312-100g
 100g 
I-1312-1g
 1g 
I-1312-25g
 25g 
I-1312-50g
 50g 
I-1312-5g
 5g 
AOB0095-10
 10mg 
AOB0095-100
 100mg 
AOB0095-25
 25mg 
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