Acrylamide for protein electrophoresis

Acrylamide for protein electrophoresis


Protein gel electrophoresis can be done on agarose gel or acrylamide gel, this type of electrophoresis is also called PAGE. The acrylamide gel is a cross-linked gel made by mixing acrylamide, which polymerises under the action of TEMED, and ammonium persulphate, which gives a more or less dense mesh network depending on the proportions of acrylamide and bis-acrylamide used, so the gel obtained behaves like a molecular sieve. The percentage of acrylamide can be the same throughout the gel or it is also possible to make a gel with a gradient (gradual percentage along the length of the gel). Loose mesh gels with large pore sizes (4-8% acrylamide) allow high molecular weight proteins to migrate more easily, whereas tight mesh gels with small pore sizes (12-20% acrylamide) limit their migration. Gradient gels therefore provide good separation resolution for both small and large proteins.

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