3D scaffold for adipocyte - Black plates (fluorescence)
BIOMIMESYS® Adipose tissue is a biofunctionalized Hyaluronic Acid (HA) hydroscaffold with components of adipose tissue extracellular matrix: collagen I, collagen VI and the cell binding domain of fibronectin (RGDS). BIOMIMESYS® Adipose tissue provides an inductive microenvironment and permits a complete differentiation of preadipocytes which are organized in an in vivo-like round shape and are dose-responsive to well-known modulators of adipocyte lipogenesis and lipolysis.
In addition to include adipose ECM components, the mechanical properties of our HA-based scaffold display a Young's modulus of 0.5 kPa, which is in the same range of adipose tissue ECM. With a pore size ranging from 70 to 170 µm, BIOMIMESYS® Adipose tissue provides also enough space for medium and nutrients flow circulation, maturation of large fragile primary adipocytes with cell-cell and cells-matrix interactions, along with a mechanical support required for an easy handling of hydroscaffold.
BIOMIMESYS® Adipose tissue increases marker genes expression of adipogenesis, in preadipocytes :
- early genes as FABP4 (fatty acid binding protein)
- late genes as GLUT4 (Glucose Transporter)
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