PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR
PCR master mix contains allcomponents for PCR, except DNA template and primers :
Taq DNA polymerase
A DNA polymerase that is heat resistant, so that it can remain intact during the DNA denaturation process and makes the apmlification of the target DNA sequence
Deoxynucleoside triphosphates (dNTPs)
dNTPs consist of four basic nucleotides : dATP, dCTP, dGTP, and dTTP. dNTPs are building blocks of new DNA strands. These four nucleotides are typically added to the PCR reaction in equimolar amounts for optimal base incorporation. In common PCR applications, the recommended final concentration of each dNTP is generally 0.2 mM.
Magnesium ion (Mg2+)
Magnesium ion (Mg2+) functions as a cofactor for activity of DNA polymerases by enabling incorporation of dNTPs during polymerization. The magnesium ions at the enzyme's active site catalyze phosphodiester bond formation between the 3′-OH of a primer and the phosphate group of a dNTP. ). In addition, Mg2+ facilitates formation of the complex between the primers and DNA templates by stabilizing negative charges on their phosphate backbones. Magnesium ion (Mg2+) are present in master mix as MgCl2 solution.
PCR is carried out in a buffer that provides a suitable chemical environment for activity of DNA polymerase. Generally a 10-50 mM Tris/HCl buffer with a pH above 8.0 (typically 8.3-8.8). KCl can be added to facilitate primer annealing, but shouldn't be higher than 50 mM as this may inhibit polymerase.
We offer a wide range of PCR master mix. Please find below a selection with main features :
|Name||Taq Mix||AceTaq Master Mix||EsayTaq PCR SuperMix|
|Product code||NB-03-0088 and NB-03-0089||P412||AS111|
|Type||Taq polymerase||Taq polymerase||Taq polymerase|
|Speed||0.9-1.2 kb/min||1 kb/min||1-2 kb/min|
|Amplicon size||< 5kb||< 5kb||< 4kb|
|5'-3' exonuclease activity||Yes||Yes||Yes|
|3'-5' exonuclease activity||No||Yes||No|
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