Hams F12 medium was originally developed for single-cell plating of near diploid cells. It is a rich modification of F10 medium designed for the cloning and serial propagation of CHD-3 and CHL-1 cell lines in the absence of serum. It also offers enhanced performance for cells growing at low density. F-12 features increased levels of choline, I-inositol, putrescine and several amino-acids. It is excellent for cultivating carcinoma cells, rat skeletal myoblasts, lung cells and rat, rabbit or chicken embryos.