Chromatrap

 Chromatrap is a more efficient, sensitive and robust method of ChIP

Compared to standard methods, Chromatrap® is:

  • FASTER
  • EASIER to use
  • MORE SENSITIVE particularly for low-abundant targets
  • Less prone to ERRORS of manual handling


Chromatrap® technology is better

Chromatrap® kits use revolutionary spin columns or microplates which contain discs of an inert, porous polymer to which protein A or G has been covalently attached. This patented format is unique. During an assay, the chromatin/antibody complex is selectively retained by the disc. Flushing with three buffers and an elution step are all that is required to obtain the selectively enriched DNA, making Chromatrap® more efficient in the laboratory:

  • ChIP in under 5 hours from chromatin preparation to qPCR
  • Less manual handling, eliminating sample loss through multiple pipetting steps
  • Single column and 96-well formats available
  • Optimised for 1000ng sample sizes: better results from smallersample
  • Great results for samples between 50ng and 3000ng

What does this mean in the laboratory?

Chromatrap® outperforms other chromatin immunoprecipitation methods:

Robust signal to noise

  • Typically 2-3 times better than standard methods
  • Low non-specific binding of the inert Chromatrap® discs
  • No pre-blocking step required
  • No DNA clean-up required

High surface area and excellent molecular mixing, ideal for:

  • Low abundant targets
  • Challenging cell types, including primary cells

Excellent ‘flow through' characteristics of the column

  • Easy, fast washing and elution steps during the assay
  • Ensures highest levels of chromatin capture and release

Wide dynamic range

  • From 50ng to 7000ng
     

Smaller sample sizes

  • More ChIP assays from a single sample

 

Web Site : www.chromatrap.com

 

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