AG Scientific

HRP Chromogens for IHC

HRP Chromogens for IHC



We offer a wide choice of specific chromogens for immunohistochemistry (IHC), for use with the 2 enzymes most used in this technique: horseradish peroxidase (HRP for Horseradish Peroxidase) and alkaline phosphatase (AP). Chromogens are chemical compounds used in IHC labeling procedures that produce a colored product that can be visualized by light microscopy. The choice of chromogen to use is directly related to the enzyme used in the test. Indeed, the enzyme will catalyze the conversion of the chromogenic substrate to produce a colored precipitate. Each enzyme can catalyze the conversion of specific substrates. Here are some examples of chromogens for HRP :

HRP:
AP:
  • Fast Red
  • Permanent Red
  • Mixture of nitro blue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP) (NBT / BCIP)

Name
Color
Mounting
Red
Aqueous
Brown
Organic
Black
Organic
Green
Organic