Vitamin B12 ELISA Kit (Bovine)
Cat# OKCA00208
Size : 96Wells
Brand : Aviva Systems Biology
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
---|
Predicted Species Reactivity | Bovine | ||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Duration | < 4 Hours | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Linearity |
| ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Vitamin B12 ELISA Kit (Bovine) (OKCA00208) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Bovine Vitamin B12 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Vitamin B12 and incubated. The Vitamin B12 found in the sample or standards competes with the biotinylated Vitamin B12 for limited binding sites on the immobilized anti-Bovine Vitamin B12 antibody. Excess unbound biotinylated Vitamin B12 and sample or standard Vitamin B12 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Vitamin B12 captured in the well and inversely proportional to the amount of Vitamin B12 which was contained in the sample or standard. | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Range | 0.312pg/mL - 20 pg/mL | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Reproducibility |
| ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||||||||||||||||||||||||||
Additional Information | CAS#: 68-19-9 Molecular Formula: C63H88CoN14O14P Molecular Weight: 1355.37 g/mol | ||||||||||||||||||||||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates | ||||||||||||||||||||||||||||||||||||||||||
Sensitivity | < 0.299 pg/mL | ||||||||||||||||||||||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of bovine VB12. No significant cross-reactivity or interference between bovine VB12 and analogues was observed. | ||||||||||||||||||||||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive Immunoassay |
Gene Symbol | Vitamin B12 |
---|---|
Alias Symbols | VB12, Vitamin B12, VB12, cobalamin |
Protein Name | vitamin B12 |
Description of Target | It is normally involved in the metabolism of every cell of the human body, especially affecting DNA synthesis and regulation, but also fatty acid synthesis and energy production. Vitamin B12, vitamin B12 or vitamin B-12, also called cobalamin, is a water-soluble vitamin with a key role in the normal functioning of the brain and nervous system, and for the formation of blood. It is one of the eight B vitamins. |