Vitamin B12 ELISA Kit (Bovine)

Cat# OKCA00208

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBovine
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration< 4 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:2091%82-99%
Serum (n=4)1:4096%91-108%
Serum (n=4)1:8093%85-97%
Serum (n=4)1:16089%82-94%
ELISA Kit PrincipleAviva Systems Biology Vitamin B12 ELISA Kit (Bovine) (OKCA00208) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Bovine Vitamin B12 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Vitamin B12 and incubated. The Vitamin B12 found in the sample or standards competes with the biotinylated Vitamin B12 for limited binding sites on the immobilized anti-Bovine Vitamin B12 antibody. Excess unbound biotinylated Vitamin B12 and sample or standard Vitamin B12 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Vitamin B12 captured in the well and inversely proportional to the amount of Vitamin B12 which was contained in the sample or standard.
ELISA Kit Range0.312pg/mL - 20 pg/mL
ELISA Kit Reproducibility
Mean Intra-Assay Precision %CVMean Inter-Assay Precision %CV
Sample123123
n202020202020
Mean (pg/mL)2.6822.662.6392.6962.592.618
SD0.0290.0530.040.0480.0610.061
CV%4.3257.7715.837.1218.7638.878
ELISA Kit Component
ComponentAmount
Anti-Vitamin B12 Microplate96 Well (12 x 8 Well Strips)
Vitamin B12 Standard 2 x 500 uL
10X Vitamin B12-Biotin Complex1 x 600 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 10 mL
Conjugate Diluent1 x 20 mL
Sample Diluent2 x 20 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional Information

CAS#: 68-19-9

Molecular Formula: C63H88CoN14O14P

Molecular Weight: 1355.37 g/mol

Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity< 0.299 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of bovine
VB12. No significant cross-reactivity or interference between bovine VB12 and
analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Gene SymbolVitamin B12
Alias SymbolsVB12, Vitamin B12, VB12, cobalamin
Protein Name vitamin B12
Description of TargetIt is normally involved in the metabolism of every cell of the human body, especially affecting DNA synthesis and regulation, but also fatty acid synthesis and energy production. Vitamin B12, vitamin B12 or vitamin B-12, also called cobalamin, is a water-soluble vitamin with a key role in the normal functioning of the brain and nervous system, and for the formation of blood. It is one of the eight B vitamins.

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