TGFB2 ELISA Kit (Bovine)

Cat# OKEH03782

Size : 96T

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TGFB2 ELISA Kit (Bovine) (OKEH03782) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TGFB2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TGFB2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TGFB2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Component
ComponentAmount
Anti-TGFB2 Microplate96 Wells (12 x 8 Well strips)
TGFB2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated TGFB2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.061 ng/mL
SpecificityNatural and recombinant Bovine Transforming growth factor beta-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTGFB2
Gene Full Nametransforming growth factor beta 2
Alias SymbolsMGF;milk growth factor;TGF-beta-2;transforming growth factor beta-2;transforming growth factor beta-2 proprotein.
NCBI Gene Id534069
Protein NameP21214
Description of TargetTransforming growth factor beta-2
Uniprot IDhttps://www.uniprot.org/uniprot/P21214
Protein Accession #NP_001106723.1
Nucleotide Accession #NM_001113252.1
Protein Size (# AA)414
Molecular Weight48 kDa

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