STAT5A ELISA Kit (Bovine)

Cat# OKEH01246

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology STAT5A ELISA Kit (Bovine) (OKEH01246) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for STAT5A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for STAT5A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample STAT5A captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 9.4% (n = 20)
ELISA Kit Component
ComponentAmount
STAT5A Microplate96 Wells (12 x 8 Well strips)
STAT5A Lyophilized Standard2
100X Biotinylated STAT5A Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.35 ng/mL
SpecificityNatural and recombinant Bovine Signal transducer and activator of transcription 5A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSTAT5A
Gene Full Namesignal transducer and activator of transcription 5A
Alias Symbolsmammary gland factor;signal transducer and activator of transcription 5A;signal transducer and activator of transcription factor 5A;STAT5A, Mammary Gland Factor;STAT5A/MGF;STATA5.
NCBI Gene Id282375
Protein NameQ95115
Description of TargetSignal transducer and activator of transcription 5A
Uniprot IDhttps://www.uniprot.org/uniprot/Q95115
Protein Accession #NP_001012691.1
Nucleotide Accession #NM_001012673.1
Protein Size (# AA)794
Molecular Weight90 kDa