SERPINC1 ELISA Kit (Bovine)

Cat# OKEH03882

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SERPINC1 ELISA Kit (Bovine) (OKEH03882) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPINC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINC1 captured in well.
ELISA Kit Range78.0-5000pg/mL
ELISA Kit Component
ComponentAmount
Anti-SERPINC1 Microplate96 Wells (12 x 8 Well strips)
SERPINC1 Lyophilized Standard2
100X Biotinylated SERPINC1 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity36 pg/mL
SpecificityNatural and recombinant Bovine Antithrombin-III
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSERPINC1
Gene Full Nameserpin family C member 1
Alias Symbolsantithrombin-III;AT3;ATIII;serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1;serpin C1.
NCBI Gene Id540261
Protein NameP41361
Description of TargetAntithrombin-III
Uniprot IDhttps://www.uniprot.org/uniprot/P41361
Protein Accession #NP_001029870.1
Nucleotide Accession #NM_001034698.2
Protein Size (# AA)465
Molecular Weight52 kDa