SERPINB6 ELISA Kit (Bovine)

Cat# OKEH02187

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SERPINB6 ELISA Kit (Bovine) (OKEH02187) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINB6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPINB6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINB6 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 9.9% (n = 20)
ELISA Kit Component
ComponentAmount
SERPINB6 Microplate96 Wells (12 x 8 Well strips)
SERPINB6 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SERPINB6 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.096 ng/mL
SpecificityNatural and recombinant Bovine Serpin B6
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSERPINB6
Gene Full Nameserpin peptidase inhibitor, clade B (ovalbumin), member 6
Alias Symbolsproteinase inhibitor 6;serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 6;serine proteinase inhibitor B-43;serpin B6.
NCBI Gene Id286854
Protein NameO02739
Description of TargetSerpin B6
Uniprot IDhttps://www.uniprot.org/uniprot/O02739
Protein Accession #NP_777214.1
Nucleotide Accession #NM_174789.1
Protein Size (# AA)378
Molecular Weight43 kDa