SAA1 ELISA Kit (Bovine)

Cat# OKEH03885

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SAA1 ELISA Kit (Bovine) (OKEH03885) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SAA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SAA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SAA1 captured in well.
ELISA Kit Range3.12-200ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 100%
ELISA Kit ReproducibilityMean Intra-Assay Precision - < 6.6%, n = 20
Mean Inter-Assay Precision - < 8.3%, n = 20
ELISA Kit Component
ComponentAmount
Anti-SAA1 Microplate96 Wells (12 x 8 Well strips)
SAA1 Lyophilized Standard2
100X Biotinylated SAA1 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity1.48 ng/mL
SpecificityNatural and recombinant Bovine Serum amyloid A protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSAA2
Gene Full Nameserum amyloid A2
Alias SymbolsSAA;SAA1;serum amyloid A protein.
NCBI Gene Id506412
Protein NameP35541
Description of TargetSerum amyloid A protein
Uniprot IDhttps://www.uniprot.org/uniprot/P35541
Protein Size (# AA)130
Molecular Weight15 kDa