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> RETN ELISA Kit (Bovine)

RETN ELISA Kit (Bovine)

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Cat# OKCD02844

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for RETN ELISA Kit (Bovine) (OKCD02844)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)96-105%90-104%87-95%85-98%
EDTA Plasma (n=5)95-103%98-105%94-105%78-89%
Heparin Plasma (n=5)95-102%80-99%82-101%78-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin (RETN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Resistin (RETN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Resistin (RETN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Resistin (RETN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)78-10493
EDTA Plasma (n=5)87-9592
Heparin Plasma(n=5)82-10485
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Resistin (RETN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Resistin (RETN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-RETN Microplate96 Wells (12 x 8 Well strips)
RETN Lyophilized Standard2
100X Biotinylated RETN Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity31 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Resistin (RETN). No significant cross-reactivity or interference between Resistin (RETN) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
  • Reconstitution & Storage Instructions
  • Western Blotting/Immunoblotting (WB/IB) Protocol
  • Immunohistochemistry (IHC) Protocol
  • Immunocytochemistry (ICC) Protocol
  • Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
  • Blocking Peptide Competition Protocol (BPCP)
  • Immunoprecipitation (IP) Protocol
  • Antibody Array (AA) Protocol
Gene SymbolRETN
Gene Full Nameresistin
Alias Symbolsadipocyte specific secreted hormone;resistin;RSTN.
NCBI Gene Id369020
Protein NameResistin
Description of TargetHormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes (By similarity).
Uniprot IDQ762I5
Protein Accession #NP_899206.1
Nucleotide Accession #NM_183362.1
Protein Size (# AA)109