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> RBP4 ELISA Kit (Bovine)

RBP4 ELISA Kit (Bovine)

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Cat# OKCD02839

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for RBP4 ELISA Kit (Bovine) (OKCD02839)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)91-99%78-90%85-93%81-92%
EDTA Plasma (n=5)94-102%92-101%90-103%83-95%
Heparin Plasma (n=5)80-99%95-102%79-94%81-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinol Binding Protein 4 (RBP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinol Binding Protein 4 (RBP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinol Binding Protein 4 (RBP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Retinol Binding Protein 4 (RBP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-9987
EDTA Plasma (n=5)88-10592
Heparin Plasma(n=5)78-9589
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinol Binding Protein 4 (RBP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinol Binding Protein 4 (RBP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-RBP4 Microplate96 Wells (12 x 8 Well strips)
RBP4 Lyophilized Standard2
100X Biotinylated RBP4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture cell supernatant and other biological fluids
Sensitivity0.141 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Retinol Binding Protein 4, Plasma (RBP4). No significant cross-reactivity or interference between Retinol Binding Protein 4, Plasma (RBP4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
  • Reconstitution & Storage Instructions
  • Western Blotting/Immunoblotting (WB/IB) Protocol
  • Immunohistochemistry (IHC) Protocol
  • Immunocytochemistry (ICC) Protocol
  • Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
  • Blocking Peptide Competition Protocol (BPCP)
  • Immunoprecipitation (IP) Protocol
  • Antibody Array (AA) Protocol
Gene SymbolRBP4
Alias SymbolsPlasma retinol-binding protein.
Protein NameRetinol-binding protein 4
Description of TargetRetinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues. Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.
Uniprot IDP18902
Protein Accession #NP_001035565.2
Nucleotide Accession #NM_001040475.2
Protein Size (# AA)183