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> PGA5 ELISA Kit (Bovine)

PGA5 ELISA Kit (Bovine)

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Cat# OKCD02799

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for PGA ELISA kit (Bovine)
Product Info
Predicted Species ReactivityBovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)99-105%83-97%93-103%94-102%
EDTA Plasma (n=5)79-98%95-103%80-103%89-98%
Heparin Plasma (n=5)98-105%89-98%85-97%78-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsinogen A (PGA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pepsinogen A (PGA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pepsinogen A (PGA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pepsinogen A (PGA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)94-10299
EDTA Plasma (n=5)80-10184
Heparin Plasma(n=5)88-10293
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pepsinogen A (PGA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pepsinogen A (PGA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PGA5 Microplate96 Wells (12 x 8 Well strips)
PGA5 Lyophilized Standard2
100X Biotinylated PGA5 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.108 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Pepsinogen A (PGA). No significant cross-reactivity or interference between Pepsinogen A (PGA) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
  • Reconstitution & Storage Instructions
  • Western Blotting/Immunoblotting (WB/IB) Protocol
  • Immunohistochemistry (IHC) Protocol
  • Immunocytochemistry (ICC) Protocol
  • Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
  • Blocking Peptide Competition Protocol (BPCP)
  • Immunoprecipitation (IP) Protocol
  • Antibody Array (AA) Protocol
Gene SymbolPGA5
Alias Symbolspga
NCBI Gene Id414350
Protein NamePepsin A
Description of TargetShows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Uniprot IDP00792
Protein Accession #NP_001001600.2
Nucleotide Accession #NM_001001600.2
Protein Size (# AA)372