NPY ELISA Kit (Bovine)
Cat# OKEH03883
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Bos taurus, Bovine | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology NPY ELISA Kit (Bovine) (OKEH03883) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-bovine NPY antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated NPY and incubated. The NPY found in the sample or standards competes with the biotinylated NPY for limited binding sites on the immobilized anti-bovine NPY antibody. Excess unbound biotinylated NPY and sample or standard NPY is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated NPY captured in the well and inversely proportional to the amount of NPY which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 31.2-2000pg/mL | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 12.8 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Bovine Pro-neuropeptide Y | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Gene Symbol | NPY |
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| Gene Full Name | neuropeptide Y |
| Alias Symbols | pro-neuropeptide Y. |
| NCBI Gene Id | 504216 |
| Protein Name | Q6RUW3 |
| Description of Target | Pro-neuropeptide Y |
| Uniprot ID | https://www.uniprot.org/uniprot/Q6RUW3 |
| Protein Accession # | NP_001014845.1 |
| Nucleotide Accession # | NM_001014845.3 |
| Protein Size (# AA) | 97 |
| Molecular Weight | 11 kDa |