MYH2 ELISA Kit (Bovine)
Cat# OKEH03871
Size : 96Wells
Brand : Aviva Systems Biology
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Bos taurus, Bovine | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology MYH2 ELISA Kit (Bovine) (OKEH03871) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-bovine MYH2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MYH2 and incubated. The MYH2 found in the sample or standards competes with the biotinylated MYH2 for limited binding sites on the immobilized anti-bovine MYH2 antibody. Excess unbound biotinylated MYH2 and sample or standard MYH2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MYH2 captured in the well and inversely proportional to the amount of MYH2 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.049 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Bovine Myosin-2 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | MYH2 |
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Gene Full Name | myosin, heavy chain 2 |
Alias Symbols | Myosin heavy chain 2;Myosin heavy chain 2a;Myosin heavy chain, skeletal muscle, adult 2. |
NCBI Gene Id | 788772 |
Protein Name | Q9BE41 |
Description of Target | Myosin-2 |
Uniprot ID | https://www.uniprot.org/uniprot/Q9BE41 |
Protein Accession # | NP_001159699.1 |
Nucleotide Accession # | NM_001166227.1 |
Protein Size (# AA) | 1940 |
Molecular Weight | 223 kDa |