MMP9 ELISA Kit (Bovine)

Cat# OKEH03849

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MMP9 ELISA Kit (Bovine) (OKEH03849) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP9 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP9 captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Component
ComponentAmount
Anti-MMP9 Microplate96 Wells (12 x 8 Well strips)
MMP9 Lyophilized Standard2
100X Biotinylated MMP9 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.57 ng/mL
SpecificityNatural and recombinant Bovine Matrix metalloproteinase-9
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMMP9
Gene Full Namematrix metallopeptidase 9
Alias Symbols92 kDa gelatinase;92 kDa type IV collagenase;gelatinase B;GELB;matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase);matrix metalloproteinase 9 (gelatinase B, 92kDa matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase);matrix metalloproteinase-9;MMP-9.
NCBI Gene Id282871
Protein NameP52176
Description of TargetMatrix metalloproteinase-9
Uniprot IDhttps://www.uniprot.org/uniprot/P52176
Protein Accession #NP_777169.1
Nucleotide Accession #NM_174744.2
Protein Size (# AA)712
Molecular Weight79 kDa