IL2RA ELISA Kit (Bovine)
Cat# OKCD02636
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for IL2RA ELISA Kit (Bovine) (OKCD02636) |
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| Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 Receptor Alpha (IL2Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 2 Receptor Alpha (IL2Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 2 Receptor Alpha (IL2Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 2 Receptor Alpha (IL2Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 15.62-1000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 2 Receptor Alpha (IL2Ra) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 2 Receptor Alpha (IL2Ra) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 6.3 pg/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Interleukin 2 Receptor Alpha (IL2Ra). No significant cross-reactivity or interference between Interleukin 2 Receptor Alpha (IL2Ra) and analogues was observed | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
| Protocol Information |
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| Gene Symbol | IL2RA |
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| Gene Full Name | interleukin 2 receptor subunit alpha |
| Alias Symbols | CD25;cytokine receptor;IL-2 receptor alpha subunit;IL-2 receptor subunit alpha;IL-2R alpha;IL-2R subunit alpha;IL2-RA;IL-2-RA;interleukin 2 receptor, alpha;interleukin-2 receptor alpha chain;interleukin-2 receptor subunit alpha;p55;TAC antigen. |
| NCBI Gene Id | 281861 |
| Protein Name | Interleukin-2 receptor subunit alpha |
| Description of Target | Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. |
| Uniprot ID | P12342 |
| Protein Accession # | NP_776783.1 |
| Nucleotide Accession # | NM_174358.2 |
| Protein Size (# AA) | 275 |