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> IFNAC Chemi-Luminescent ELISA Kit (Bovine)

IFNAC Chemi-Luminescent ELISA Kit (Bovine)

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Cat# OKCD03575

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for IFNAC Chemi-Luminescent ELISA Kit (Bovine) (OKCD03575)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)78-104%79-96%81-96%97-105%
EDTA Plasma (n=5)85-99%93-102%80-97%93-101%
Heparin Plasma (n=5)78-90%96-103%95-102%83-101%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha (IFNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha (IFNa) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)99-105102
EDTA Plasma (n=5)96-10499
Heparin Plasma (n=5)85-10188
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Ifnac Microplate96 Wells (12 x 8 Well strips)
Ifnac Lyophilized Standard2
100X Ifnac Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.6 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIfnac
Alias SymbolsIF1AG7
NCBI Gene Id281236
Protein NameInterferon alpha-1
Description of TargetProduced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Uniprot IDP07348
Protein Accession #NP_776510
Nucleotide Accession #NM_174085