GSN ELISA Kit (Bovine)

Cat# OKEH00403

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GSN ELISA Kit (Bovine) (OKEH00403) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Bovine GSN antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Bovine GSN and incubated. The Bovine GSN found in the sample or standards competes with the biotinylated Bovine GSN for limited binding sites on the immobilized anti-Bovine GSN antibody. Excess unbound biotinylated Bovine GSN and sample or standard Bovine GSN is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Bovine GSN captured in the well and inversely proportional to the amount of Bovine GSN which was contained in the sample or standard.
ELISA Kit Range3.12-200ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-GSN Microplate96 Wells (12 x 8 Well strips)
GSN Lyophilized Standard2
Sample Diluent1 x 20 mL
100X GSN-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity1.5 ng/mL
SpecificityNatural and recombinant Bovine Gelsolin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolGSN
Gene Full Namegelsolin
Alias Symbolsactin-depolymerizing factor;ADF;brevin;gelsolin;gelsolin (amyloidosis, Finnish type).
NCBI Gene Id535077
Protein NameQ3SX14
Description of TargetGelsolin
Uniprot IDhttps://www.uniprot.org/uniprot/Q3SX14
Protein Accession #NP_001029799.1
Nucleotide Accession #NM_001034627.1
Protein Size (# AA)731
Molecular Weight80 kDa