CCL8 ELISA Kit (Bovine)
Cat# OKCD02719
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for CCL8 ELISA Kit (Bovine) (OKCD02719) |
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| Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 2 (MCP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 2 (MCP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monocyte Chemotactic Protein 2 (MCP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Monocyte Chemotactic Protein 2 (MCP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 15.62-1000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 2 (MCP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 2 (MCP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture cell supernatant and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 6.4 pg/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Monocyte Chemotactic Protein 2 (MCP2). No significant cross-reactivity or interference between Monocyte Chemotactic Protein 2 (MCP2) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
| Protocol Information |
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| Gene Symbol | CCL8 |
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| Gene Full Name | chemokine (C-C motif) ligand 8 |
| Alias Symbols | C-C motif chemokine 8;C-C motif chemokine 8-like;MCP-2;monocyte chemoattractant protein 2;monocyte chemotactic protein 2;SCYA8;small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2);small-inducible cytokine A8. |
| NCBI Gene Id | 281044 |
| Protein Name | C-C motif chemokine 8 |
| Description of Target | Chemotactic factor that attracts monocytes. This protein can bind heparin. |
| Uniprot ID | Q09141 |
| Protein Accession # | NP_776432.1 |
| Nucleotide Accession # | NM_174007.1 |
| Protein Size (# AA) | 99 |