C4 ELISA Kit (Bovine)

Cat# OKEH03837

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology C4 ELISA Kit (Bovine) (OKEH03837) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C4 captured in well.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Component
ComponentAmount
Anti-C4 Microplate96 Wells (12 x 8 Well strips)
C4 Lyophilized Standard2
100X Biotinylated C4 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity1.49 ng/mL
SpecificityNatural and recombinant Bovine Complement C4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolC4
Gene Full Namecomplement component 4A
Alias SymbolsC4
NCBI Gene Id280678
Protein NameP01030
Description of TargetComplement C4
Uniprot IDhttps://www.uniprot.org/uniprot/P01030
Protein Accession #NP_001159957.1
Nucleotide Accession #NM_001166485.1
Protein Size (# AA)920
Molecular Weight102 kDa