Specifications

Product Data
Clone Name	9A6
Applications	ELISA, FC, IF, WB
Recommended Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml. Flow Cytometry. Immunocytochemistry.
Reactivities	Human, Mouse
Host	Mouse
Isotype	IgG3
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjuagted to KLH
Specificity	This antibody specifically interacts with activated src phosphorylated at Tyr 416 and with src family kinases phosphorylated at the corresponding Tyr in the activation loop. The antibody does not crossreact with the non-phosphorylated form of src nor with unrelated phosphorylation sites.
Formulation	0.5 mg/ml in PBS/0.09% Na-Azide/PEG and Sucrose. Label: Biotin State: Liquid purified IgG fraction.
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Biotin
Storage	Store the antibody (Aliquote and freeze in liquid nitrogen)at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Stability	Shelf life: one year from despatch.
Database Link	Entrez Gene 6714 Human UniProt ID P12931
Background	Src is the best understood member of a family of nine tyrosine kinases regulating cellular responses to extracellular stimuli. Src becomes activated upon dephosphorylation of Tyr 527, leading to a conformational change of the src molecule towards an open, active conformation which allows binding to other proteins via the SH2 and SH3 domain. Activated src phosphorylates a variety of substrates involved in the mitogenic signalling pathway, cytoskeletal organization, cell-substrate adhesion, cell-cell adhesion, cell-cell communication, and RNA processing.
Synonyms	c-Src, pp60c-src
Note	Molecular weight of antigen: 60 kDa
Reference Data