Rat RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit

Cat# NB-22-42073-1

Size : 96T

Brand : Neo Biotech

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Phone : +1 850 650 7790

  • Rat RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit

General Info

Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This rat RBP4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence

Information

Gene Symbol: Rbp4
Gene ID: 25703
Uniprot ID: RET4_RAT
Specificity: This kit recognizes Rat RBP4 in samples. No significant cross-reactivity or interference between Rat RBP4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids

Description

Tissue Specificity Detected in blood plasma (at protein level).
Function Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues. Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.
Protein Name Retinol-Binding Protein 4
Plasma Retinol-Binding Protein
Prbp
Rbp
Database Links Reactome: R-RNO-2453902
Reactome: -RNO-975634
Cellular Localisation Secreted
Alternative CLIA Names Retinol-Binding Protein 4 CLIA kit
Plasma Retinol-Binding Protein CLIA kit
Prbp CLIA kit
Rbp CLIA kit
Rbp4 CLIA kit
Specificity This kit recognizes Rat RBP4 in samples. No significant cross-reactivity or interference between Rat RBP4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-102 97
EDTA plasma(n=8) 101-116 106
Cell culture media(n=8) 86-100 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.92 2.69 8.21 0.93 2.65 8.40
Standard deviation 0.09 0.20 0.82 0.08 0.26 0.88
CV (%) 9.78 7.43 9.99 8.60 9.81 10.48
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance