General Info
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.19ng/mL |
| Detection Limit: | 0.31~20ng/mL |
| Short Description: | This human RBP4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
Information
| Gene Symbol: | RBP4 |
| Gene ID: | 5950 |
| Uniprot ID: | RET4_HUMAN |
| Specificity: | This kit recognizes Human RBP4 in samples. No significant cross-reactivity or interference between Human RBP4 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Tissue Specificity | Detected in blood plasma and in urine (at protein level). |
| Function | Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane. |
| Protein Name | Retinol-Binding Protein 4Plasma Retinol-Binding ProteinPrbpRbp Cleaved Into - Plasma Retinol-Binding Protein(1-182 - Plasma Retinol-Binding Protein(1-181 - Plasma Retinol-Binding Protein(1-179 - Plasma Retinol-Binding Protein(1-176 |
| Database Links | Reactome: R-HSA-2453864Reactome: R-HSA-2453902Reactome: R-HSA-6809583Reactome: R-HSA-975634 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Retinol-Binding Protein 4 CLIA kitPlasma Retinol-Binding Protein CLIA kitPrbp CLIA kitRbp Cleaved Into - Plasma Retinol-Binding Protein(1-182 - Plasma Retinol-Binding Protein(1-181 - Plasma Retinol-Binding Protein(1-179 - Plasma Retinol-Binding Protein(1-176 CLIA kitRBP4 CLIA kitPRO2222 CLIA kit |
| Specificity | This kit recognizes Human RBP4 in samples. No significant cross-reactivity or interference between Human RBP4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 102-113 | 108 |
| EDTA plasma(n=8) | 87-100 | 93 |
| Cell culture media(n=8) | 98-112 | 105 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.94 | 2.72 | 7.42 | 0.88 | 2.55 | 6.87 |
| Standard deviation | 0.10 | 0.28 | 0.54 | 0.11 | 0.19 | 0.43 |
| CV (%) | 10.64 | 10.29 | 7.28 | 12.50 | 7.45 | 6.26 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance