Oxaloacetate BioAssay™ Kit (OAA)

Cat# 172379-100T

Size : 100Tests

Brand : US Biological

Contact local distributor :

Phone : +1 850 650 7790

Shipping Temp
Blue Ice

Storage Temp

Oxaloacetate (OAA) is an intermediate in the citric acid cycle and participates in gluconeogenesis. OAA is formed by the oxidation of malate, by deamidation of aspartate or by condensation of CO2 with pyruvate or phosphoenolpyruvate. The Oxaloacetate Assay Kit provides a simple, direct and automation-ready procedure for measuring oxaloacetate concentration. OAA is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570nm or
fluorescence intensity at lex/em = 530/585nm is directly proportional to the oxaloacetate concentration in the sample.

Intended Use:
For quantitative determination of oxaloacetate and evaluation of drug effects on its metabolism.

Direct Assays: oxaloacetate in plasma, serum, tissue and culture media.

7-400um oxaloacetate for colorimetric assays and 1-40um for fluorometric assays.

Detection limit:
OD, FL: 4, 1um

Kit Components:
Developer, 1x10ml
Dye Reagent, 120ul
ODC Enzyme, 1x120ul
Oxaloacetate Standard, 1xdried

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.