Isocitrate BioAssay™ Kit (Isocitrate Acid)
Cat# 172373-100T
Size : 100Tests
Brand : US Biological
Contact local distributor :
Phone : +1 850 650 7790
Shipping Temp
Blue IceStorage Temp
4°C/-20°CIsocitrate (Isocitrate Acid) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing alpha-ketoglutarate and generating NADPH. Isocitrate is commonly
found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. The isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly
fluorescent product. The fluorescence intensity of this product, measured at lex/em= 530/585nm, is proportional to the isocitrate concentration in the sample.
found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. The isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly
fluorescent product. The fluorescence intensity of this product, measured at lex/em= 530/585nm, is proportional to the isocitrate concentration in the sample.
Intended Use:
For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.
Applications:
Isocitrate determination in food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Range:
0.6-500um for 10 min reaction.
Detection limit:
0.6um
Kit Components:
Assay Buffer, 1x10ml
NADP, 1x500ul
Standard, 1x1ml
Enzyme A, 1x120ul
Enzyme B, 1x120ul
Probe, 1x750ul
NADP, 1x500ul
Standard, 1x1ml
Enzyme A, 1x120ul
Enzyme B, 1x120ul
Probe, 1x750ul
Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.