Glutathione Peroxidase (GSH-PX) BioAssay™ Kit
Cat# 405784-1Kit
Size : 1Kit
Brand : US Biological
Shipping Temp
Blue IceStorage Temp
4°C/-20°CGlutathione Peroxidase (GSH-PX) is an important enzyme that catalyzes decomposition of hydrogen peroxide. GSH specifically catalyze the reaction between GSH and hydrogen peroxide, protecting cell membrane structure and keeping membrane function integrity. Se-cysteine is the active center of the GSH-PX. Determination of GSH-PX activity in organism can be an indicator of selenium level as Se is essential section of GSH-PX.
Sample Type:
Serum, plasma, tissue, whole blood, red blood cell
Intended Use:
This kit can be used to measure GSH-PX activity of animal serum, plasma, tissue, whole blood, red blood cell and other samples.
Kit Components (see Protocol for final kit quantities):
Reagent 1,
Sample homogenate, 50mL
Sample homogenate, 50mL
Reagent 2,
GSH, 15.4mg
GSH, 15.4mg
Reagent 3,
GSH preparation solution, 10mL
GSH preparation solution, 10mL
Reagent 4,
Oxidant, 2×1 mL
Oxidant, 2×1 mL
Reagent 5,
Acidic precipitation agent, 50mL
Reagent 6,
GSH-Px assay buffer, 15mL
Acidic precipitation agent, 50mL
Reagent 6,
GSH-Px assay buffer, 15mL
Reagent 7,
Benzoic acid chromogenic solution, 3mL
Benzoic acid chromogenic solution, 3mL
Reagent 8,
ddH2O, 50mL
ddH2O, 50mL
Manual, 1xcopy
Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards