Total Glutathione/Oxidized Glutathione BioAssay™ Kit

Cat# 405829-96T

Size : 96Tests

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Shipping Temp
Blue Ice

Storage Temp
4°C/-20°C

Glutathione is widely distributed in the body. It plays a direct or indirect role in many important biological phenomena, such as mechanism of enzyme reaction, synthesis of biomacromolecules (such as protein and DNA), intermediate metabolism, radiation, tumor, immune, environmental toxins, aging and so on.

The Total Glutathione/Oxidized Glutathione BioAssay™ Kit can be used to measure total glutathione (T-GSH) and oxidized glutathione (GSSG) content in serum, plasma, animal tissue, whole blood, red blood cells and cultured cells samples.

Sensitivity:
0.12umol/L T-GSH

Detection Range:
0.12-30umol/L T-GSH

Test Principle:
GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.

Kit Components:
Reagent 1,
Buffer Solution, 3x55ml
Reagent 2,
GSSG Standard, 1x6.13mg
Reagent 3,
Protein Precipitator, 1xVial
Reagent 4,
Chromogenic Agent
Reagent 5,
Chromogenic Agent, 2xVials
Reagent 6,
Diluent, 1x3.6ml
Reagent 7,
GSH Scavenger Auxiliary Solution, 1x1.4ml
Reagent 8,
GSH Scavenger, 1x200ul
Reagent 9,
Substrate, 2xVials
Reagent 10,
Stop Solution, 1x50ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards