Glucose-6-Phosphate Dehydrogenase BioAssay™ Kit (G6PDH)

Cat# 172370-100T

Size : 100Tests

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Shipping Temp
Blue Ice

Storage Temp
4°C/-20°C

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. The non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565nm. The increase in absorbance at 565nm is proportional to the enzyme
activity.

Intended Use:
For Quantitative colorimetric kinetic determination of Glucose-6-Phosphate Dehydrogenase activity and evaluation of drug effects on its metabolism.

Applications:
G6PDH activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)

Range:
0.2-100U/L for 15 min reaction.

Kit Components:
Assay Buffer, 1x10ml
Diaphorase, 1x120ul
NADP/MTT, 1x1ml
Calibrator, 1x10ml
Substrate, 1x1ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.