Mouse Anti-Human CD45 [Clone CD45-Ab-13]

Background Information​

CD45, also known as leukocyte common antigen (LCA), protein tyrosine phosphatase, receptor type I (PTPR) is a type I transmembrane protein encoded by PTPR gene specifically expresses in hematopoietic cells. PRPR gene has 34 exons. Dfferentially spliced transcription of exon 4, 5 and 6 yields 6 isoforms: ABC (exon 4, 5 and 6), AB (exon 4 and 5) or BC (Exon 5 and 6), A (only exon 4), B (only exon 5) and O (no exon 4, 5, or 6). Conventional monoclonal antibodies(mAbs) raised against CD45 have reactivity against all isoforms, while restricted mAB only reacts to specific isotypes (CD45RA, CD45RB, CD45RO). BMI-CD45Ab13, identifies all three major subsets of  leukocyte (Granulocyte, monocyte and lymphocyte) and was selected by Professor Leon Terstappen to participate in CANCER-ID Consortium.

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Applications

This antibody is useful in Flow Cytometry, Enzyme-Linked Immuno Sorbent Assay, Western Blot, Immunocytochemistry, Immnofluorescence.

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Product Details

• Product name:  Anti-CD45 Monoclonal Antibody

• Catalog number: BMI-CD45Ab13

• Size: 100ug

• Gene: CD45

• Purity: IgG purified

• Isotype: IgG2b

• Immunogen: Purified CD45

• Localization: Cell membrane

• Host: Mouse

• Clone: CD45-Ab-13

• Clonality: Monoclonal

• Category: Primary

• Target species: Human

• Specificity:  This antibody has specificity for CD45

• Species specificity: Cross-reacts with Human. Does not cross react with mouse

• Dilution range: Flow Cytometry, Enzyme-Linked ImmunoSorbent Assay 0.01~1 μg/ml,

• Western Blot, Immunocytochemistry, Immnofluorescence 0.1~1 μg/ml

• Form:  Liquid ( PBS (pH 7.4) with 0.09% NaN3)

• Conjugate: unconjugated

• Shipping and storage: 2-8°C, DO NOT FREEZE