Vitamin A ELISA Kit

Cat# OKEH02573

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02573
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Vitamin A ELISA Kit (OKEH02573) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Vitamin A antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Vitamin A and incubated. The Vitamin A found in the sample or standards competes with the biotinylated Vitamin A for limited binding sites on the immobilized anti-Vitamin A antibody. Excess unbound biotinylated Vitamin A and sample or standard Vitamin A is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Vitamin A captured in the well and inversely proportional to the amount of Vitamin A which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.2% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Vitamin A Microplate96 Wells (12 x 8 Well strips)
Vitamin A Lyophilized Standard2 x 10 ng
Sample Diluent1 x 20 mL
100X Vitamin A-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: VA
Target Name: Vitamin A
::Pubchem: 445354
::Chemical formula: C20H30O
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.052 ng/mL
SpecificityNatural and recombinant General Vitamin A
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Description
Alias SymbolsVA, Vitamin A, VA
Protein NameVitamin A
Description of TargetVitamin A (or Vitamin A Retinol, retinal, and four carotenoids including beta carotene) is a vitamin that is needed by the retina of the eye in the form of a specific metabolite, the light-absorbing molecule retinal, that is necessary for both low-light (scotopic vision) and color vision. Vitamin A also functions in a very different role as an irreversibly oxidized form of retinol known as retinoic acid, which is an important hormone-like growth factor for epithelial and other cells.