Tetrahydrofolic Acid ELISA Kit

Cat# OKCD02291

Size : 96Wells

Brand : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Tetrahydrofolic Acid ELISA Kit (OKCD02291)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)89-96%91-98%82-102%78-102%
EDTA plasma(n=5)92-102%88-97%87-101%97-104%
heparin plasma(n=5)88-97%80-97%89-101%88-101%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tetrahydrofolic Acid (THFA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tetrahydrofolic Acid (THFA) and unlabeled Tetrahydrofolic Acid (THFA) (Standards or samples) with the pre-coated antibody specific to Tetrahydrofolic Acid (THFA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tetrahydrofolic Acid (THFA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tetrahydrofolic Acid (THFA) in the sample.
ELISA Kit Range1.23-100ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)89-9792
EDTA plasma(n=5)91-9996
heparin plasma(n=5)91-9996
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tetrahydrofolic Acid (THFA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tetrahydrofolic Acid (THFA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Tetrahydrofolic Acid Microplate96 Wells (12 x 8 Well Strips)
Tetrahydrofolic Acid Lyophilized Standard2
100X Tetrahydrofolic Acid Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Additional InformationCAS: 135-16-0
PubChem: 91443
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity0.52 ng/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolTHFA
Alias SymbolsTetrahydrofolic acid; Tetrahydrofolate; 5,6,7,8-tetrahydrofolic acid; 135-16-0; Tetrahydropteroylglutamic acid
Protein NameTetrahydrofolic Acid
Description of TargetTetrahydrofolic acid is a folic acid derivative. It is produced from dihydrofolic acid by dihydrofolate reductase. It is converted into 5,10-methylenetetrahydrofolate by serine hydroxymethyltransferase. It is a coenzyme in many reactions, especially in the metabolism of amino acids and nucleic acids. It acts as a donor of a group with one carbon atom. It gets this carbon atom by sequestering formaldehyde produced in other processes.