Tetrahydrobiopterin ELISA Kit

Cat# OKEH02612

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Tetrahydrobiopterin ELISA Kit (OKEH02612) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Tetrahydrobiopterin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tetrahydrobiopterin and incubated. The Tetrahydrobiopterin found in the sample or standards competes with the biotinylated Tetrahydrobiopterin for limited binding sites on the immobilized anti-Tetrahydrobiopterin antibody. Excess unbound biotinylated Tetrahydrobiopterin and sample or standard Tetrahydrobiopterin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tetrahydrobiopterin captured in the well and inversely proportional to the amount of Tetrahydrobiopterin which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.9% (n = 20)
Mean Inter-assay CV%: < 8.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Tetrahydrobiopterin Microplate96 Wells (12 x 8 Well strips)
Tetrahydrobiopterin Lyophilized Standard2 x 50 uIU
Sample Diluent1 x 20 mL
100X Tetrahydrobiopterin-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: THB
Target Name: Tetrahydrobiopterin
::Pubchem: 44257
::Chemical formula: C9H15N5O3
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.067 ng/mL
SpecificityNatural and recombinant General Tetrahydrobiopterin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsTHB, Tetrahydrobiopterin, THB, BH4
Protein NameTetrahydrobiopterin
Description of TargetTetrahydrobiopterin (BH4, THB, trade name Kuvan) or sapropterin (INN) is a naturally occurring essential cofactor of the three aromatic amino acid hydroxylase enzymes, used in the degradation of amino acid phenylalanine and in the biosynthesis of the neurotransmitters serotonin (5-hydroxytryptamine, 5-HT), melatonin, dopamine, norepinephrine (noradrenaline), epinephrine (adrenaline), and is a cofactor for the production of nitric oxide (NO) by the nitric oxide synthases.

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